Selectivity of plasma membrane calcium ATPase (PMCA)-mediated extrusion of toxic divalent cations in vitro and in cultured cells (2024)

Mariela Ferreira Gomes

2017, Archives of toxicology

In the recent years, the toxicity of certain divalent cations has been associated with the alteration of intracellular Ca(2+) homeostasis. Among other mechanisms, these cations may affect the functionality of certain Ca(2+)-binding proteins and/or Ca(2+) pumps. The plasma membrane calcium pump (PMCA) maintains Ca(2+) homeostasis in eukaryotic cells by mediating the efflux of this cation in a process coupled to ATP hydrolysis. The aim of this work was to investigate both in vitro and in cultured cells if other divalent cations (Sr(2+), Ba(2+), Co(2+), Cd(2+), Pb(2+) or Be(2+)) could be transported by PMCA. Current results indicate that both purified and intact cell PMCA transported Sr(2+) with kinetic parameters close to those of Ca(2+) transport. The transport of Pb(2+) and Co(2+) by purified PMCA was, respectively, 50 and 75% lower than that of Ca(2+), but only Co(2+) was extruded by intact cells and to a very low extent. In contrast, purified PMCA-but not intact cell PMCA-transpor...

Related papers

Transport parameters and stoichiometry of active calcium ion extrusion in intact human red cells

Balazs Sarkadi

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1977

View PDFchevron_right

Physiologic rate of carrier-mediated Ca2+ entry matches active extrusion in human erythrocytes

Paul Schlesinger

The Journal of General Physiology, 1991

View PDFchevron_right

The role of plasma membrane CA2+ Pumps (PMCAs) in pathologies of mammalian cells

Jan Lehotský

Frontiers in Bioscience, 2002

View PDFchevron_right

The plasma membrane Ca²+ ATPase and the plasma membrane sodium calcium exchanger cooperate in the regulation of cell calcium

Ernesto Carafoli

Cold Spring Harbor perspectives in biology, 2011

Calcium is an ambivalent signal: it is essential for the correct functioning of cell life, but may also become dangerous to it. The plasma membrane Ca(2+) ATPase (PMCA) and the plasma membrane Na(+)/Ca(2+) exchanger (NCX) are the two mechanisms responsible for Ca(2+) extrusion. The NCX has low Ca(2+) affinity but high capacity for Ca(2+) transport, whereas the PMCA has a high Ca(2+) affinity but low transport capacity for it. Thus, traditionally, the PMCA pump has been attributed a housekeeping role in maintaining cytosolic Ca(2+), and the NCX the dynamic role of counteracting large cytosolic Ca(2+) variations (especially in excitable cells). This view of the roles of the two Ca(2+) extrusion systems has been recently revised, as the specific functional properties of the numerous PMCA isoforms and splicing variants suggests that they may have evolved to cover both the basal Ca(2+) regulation (in the 100 nM range) and the Ca(2+) transients generated by cell stimulation (in the μM ran...

View PDFchevron_right

Characterization of PMCA isoforms and their contribution to transcellular Ca<sup>2+</sup>flux in MDCK cells

Sertac Kip

American Journal of Physiology-renal Physiology, 2003

View PDFchevron_right

Plasma membrane Ca2+-ATPases can shape the pattern of Ca2+ transients induced by store-operated Ca2+ entry

Emanuel Strehler, Agnes Enyedi

Science Signaling, 2015

View PDFchevron_right

The plasma membrane calcium pump in health and disease

Marisa Brini

FEBS Journal, 2013

View PDFchevron_right

Plasma Membrane Ca2+ ATPases as Dynamic Regulators of Cellular Calcium Handling

Emanuel Strehler, Agnes Enyedi

Annals of the New York Academy of Sciences, 2007

View PDFchevron_right

The effect of extracellularly applied divalent cations on cytosolic Ca2+ in murine Leydig cells: evidence for a Ca2+‐sensing receptor

Joseph Igietseme

The Journal of …, 1998

View PDFchevron_right

The effect of cellular calcium on Na+/K+ cotransport in human red blood cells

georges dagher

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1987

View PDFchevron_right

Related papers

Ca2+ transport by rat liver plasma membranes: the transporter and the previously reported Ca2+-ATPase are different enzymes

Alan Dawson

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1988

View PDFchevron_right

Equilibrium binding of calcium to fragmented human red cell membranes and its relation to calcium-mediated effects on cation permeability

Hartmut Porzig

The Journal of Membrane Biology, 1978

View PDFchevron_right

Calcium and cancer: targeting Ca2+ transport

Greg Monteith

Nature Reviews …, 2007

View PDFchevron_right

Active calcium transport via coupling between the enzymatic and the ionophoric sites of Ca/sup 2 +/ + Mg/sup 2 +/-ATPase

Adil Shamoo

1977

View PDFchevron_right

Capacitative calcium influx in human epithelial breast cancer and non-tumorigenic cells occurs through Ca2+ entry pathways with different permeabilities to divalent cations

Carolina Baldi

Journal of Cellular Biochemistry, 2003

View PDFchevron_right

Complete primary structure of a human plasma membrane Ca2+ pump

Emanuel Strehler

Journal of Biological Chemistry, 1988

View PDFchevron_right

Primary Active Ca2+ Transport Systems in Health and Disease

Peter Vangheluwe

Cold Spring Harbor Perspectives in Biology, 2019

View PDFchevron_right

Sodium influx via a non-selective pathway activated by the removal of extracellular divalent cations: possible role in the calcium paradox

Kanigula Mubagwa

Cardiovascular Research, 1999

View PDFchevron_right

Role of Oxidant Scavengers in the Prevention of Ca2+ Homeostasis Disorders

I. Jardin

Molecules, 2010

View PDFchevron_right

Calcium Occlusion in Plasma Membrane Ca2+-ATPase

R. Gonzalez-lebrero

Journal of Biological Chemistry, 2011

View PDFchevron_right

Plasma membrane calcium ATPases: From generic Ca(2+) sump pumps to versatile systems for fine-tuning cellular Ca(2.)

Emanuel Strehler

Biochemical and biophysical research communications, 2015

View PDFchevron_right

Effects of divalent metal ions on the calcium pump and membrane phosphorylation in human red cells

Balazs Sarkadi, Agnes Enyedi

View PDFchevron_right

The plasma membrane calcium pump: not just another pretty ion translocase

Thomas Vanaman

Glycobiology, 1996

View PDFchevron_right

The plasma membrane Ca 2+ ATPase of animal cells: Structure, function and regulation

Dmitry Lim

Archives of Biochemistry and Biophysics, 2008

View PDFchevron_right

Inhibition of the renal betaine transporter by calcium ions

Michael Sturek

American Journal of Physiology-renal Physiology, 2006

View PDFchevron_right

A Comparative Functional Analysis of Plasma Membrane Ca2+ Pump Isoforms in Intact Cells

Marisa Brini

Journal of Biological Chemistry, 2003

View PDFchevron_right

PHOSPHORYLATION OF THE Ca 2+ PUMP INTERMEDIATE IN INTACT RED CELLS, ISOLATED MEMBRANES AND INSIDE-OUT VESICLES

Balazs Sarkadi

Mol Cell Biochem, 1978

View PDFchevron_right

Activation of the human red cell calcium ATPase by calcium pretreatment

Pedro J. Romero

The Journal of Membrane Biology, 1994

View PDFchevron_right

Intracellular acidification associated with changes in free cytosolic calcium. Evidence for Ca2+/H+ exchange via a plasma membrane Ca(2+)-ATPase in vascular smooth muscle cells

Guillermo Flores

Journal of Clinical Investigation, 1995

View PDFchevron_right

Active calcium transport via coupling between the enzymatic and the ionophoric sites of Ca2+ + Mg2+-ATPase

Adil Shamoo

Journal of Supramolecular Structure, 1977

View PDFchevron_right

Multifaceted plasma membrane Ca(2+) pumps: From structure to intracellular Ca(2+) handling and cancer

Ágnes Enyedi

Biochimica et biophysica acta, 2015

View PDFchevron_right

Effects of PMCA and SERCA pump overexpression on the kinetics of cell Ca2+ signalling

Stefano Manni

The EMBO journal, 2000

View PDFchevron_right

Related topics

BiochemistryBiophysicsChemistryCalciumCell BiologyMedicineATPaseMembraneCiencias BiológicasCalcium HomeostasisHomeostasisIon TransportEnseñanza - Aprendizaje Ciencias...IntracellularPharmacology and pharmaceutical ...

Selectivity of plasma membrane calcium ATPase (PMCA)-mediated extrusion of toxic divalent cations in vitro and in cultured cells (2024)

References

Top Articles
Latest Posts
Recommended Articles
Article information

Author: Annamae Dooley

Last Updated:

Views: 6406

Rating: 4.4 / 5 (45 voted)

Reviews: 84% of readers found this page helpful

Author information

Name: Annamae Dooley

Birthday: 2001-07-26

Address: 9687 Tambra Meadow, Bradleyhaven, TN 53219

Phone: +9316045904039

Job: Future Coordinator

Hobby: Archery, Couponing, Poi, Kite flying, Knitting, Rappelling, Baseball

Introduction: My name is Annamae Dooley, I am a witty, quaint, lovely, clever, rich, sparkling, powerful person who loves writing and wants to share my knowledge and understanding with you.