Mariela Ferreira Gomes
2017, Archives of toxicology
In the recent years, the toxicity of certain divalent cations has been associated with the alteration of intracellular Ca(2+) homeostasis. Among other mechanisms, these cations may affect the functionality of certain Ca(2+)-binding proteins and/or Ca(2+) pumps. The plasma membrane calcium pump (PMCA) maintains Ca(2+) homeostasis in eukaryotic cells by mediating the efflux of this cation in a process coupled to ATP hydrolysis. The aim of this work was to investigate both in vitro and in cultured cells if other divalent cations (Sr(2+), Ba(2+), Co(2+), Cd(2+), Pb(2+) or Be(2+)) could be transported by PMCA. Current results indicate that both purified and intact cell PMCA transported Sr(2+) with kinetic parameters close to those of Ca(2+) transport. The transport of Pb(2+) and Co(2+) by purified PMCA was, respectively, 50 and 75% lower than that of Ca(2+), but only Co(2+) was extruded by intact cells and to a very low extent. In contrast, purified PMCA-but not intact cell PMCA-transpor...
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